Journal: Scientific Reports

Article Title: Proteomic analysis of plasma proteins of high-flux haemodialysis and on-line haemodiafiltration patients reveals differences in transthyretin levels related with anaemia

doi: 10.1038/s41598-020-72104-5

Figure Lengend Snippet: Transthyretin (TTR) detection by western blot in HF and HDF plasma samples. Individual albumin- and IgG-depleted plasma samples of each HF and HDF patient were analysed independently by western blotting with anti-TTR antibody (images). In the images, arrows show the TTR protein detected, and the numbers on the left indicate the apparent molecular mass from standards. The box graph show the quantification of the TTR levels (arbitrary units, A.U.) detected in HF and HDF samples represented as the mean of the 10 and 9 samples, respectively, (thick line) ± 25% and 75% percentile (box) and the minimum and maximum TTR level (whiskers). * p < 0.05, HF compared with HDF by t-test. Images show whole blots. Blotted proteins were staining with Fast Green as loading control of the analyzed samples, and used for normalization of detected TTR levels (see Figure in the Supplementary Material).

Article Snippet: The membranes were incubated overnight at 4 °C with rabbit polyclonal anti-TTR antibody (ab78548, Abcam), washed, then incubated for 1 h with peroxidase-conjugated anti-rabbit IgG (GE Healthcare), and developed with Clarity reagent (Bio-Rad).

Techniques: Western Blot, Clinical Proteomics, Staining, Control

Journal: STAR Protocols

Article Title: Generation of mouse hippocampal brain organoids from primary embryonic neural stem cells

doi: 10.1016/j.xpro.2023.102413

Figure Lengend Snippet: List of primary antibodies and related target used to perform immunofluorescence analysis on brain organoids

Article Snippet: PREALBUMIN (TTR) (Genetex, #GTX85112) , Choroid plexus.

Techniques: Immunofluorescence, Produced

Journal: STAR Protocols

Article Title: Generation of mouse hippocampal brain organoids from primary embryonic neural stem cells

doi: 10.1016/j.xpro.2023.102413

Figure Lengend Snippet:

Article Snippet: PREALBUMIN (TTR) (Genetex, #GTX85112) , Choroid plexus.

Techniques: Recombinant, Protease Inhibitor, Bicinchoninic Acid Protein Assay, Calcium Assay, Software, Imaging, Microscopy, Spectrophotometry, Real-time Polymerase Chain Reaction

Journal: Mediators of Inflammation

Article Title: Plasma Proteome Profiling of Coronary Artery Disease Patients: Downregulation of Transthyretin—An Important Event

doi: 10.1155/2020/3429541

Figure Lengend Snippet: Validation of TTR in CAD Plasma: (a) The enzyme-linked immunosorbent assay of TTR from plasma of CAD patients ( n = 200) and control ( n = 50) indicated significant low level with a 1.61-fold p < 0.0001. (b) The ROC plot indicates the individual protein abundance of each group with area under curve AUC: 0.930. The statistical significance was determined by Student's t -test, p < 0.05.

Article Snippet: The enzyme-linked immunosorbent assay (ELISA) was carried out using anti-TTR antibody (ProSci, USA).

Techniques: Biomarker Discovery, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Control, Quantitative Proteomics

Journal: bioRxiv

Article Title: 3D Visualization and Proteomic Analysis of Human Cardiac Transthyretin Amyloidosis Tissue Reveals Microangiopathy and Capillary Occlusion

doi: 10.64898/2026.02.09.704431

Figure Lengend Snippet: Optically-cleared left ventricular free wall tissue reveals disorganized angiogenesis. The organized lattice vascular structure observed in the age-matched control via Collagen IV staining (red) is lost in favor of hypervascular regions (black arrows) and hypovascular regions (white arrows). Non-native TTR (green) appears to replace collagen IV (yellow arrows) on the border of amyloid-rich hypovascular regions. Amyloid is stained with AmyTracker 480 (blue). Protego’s 5D3 antibody targets a TTR sequence (TSESGELHGLTTE) that is covered in its native tetramer conformation, thereby only binding to non-native forms of TTR. Non-native TTR overlaps with AmyTracker 480 on the margins of amyloid deposits, suggesting a dense fibrillar core (blue) surrounded by nonfibrillar non-native TTR aggregates (green).

Article Snippet: To study antibody binding of amyloids, a suspension of ATTR fibril-containing extract (100 μL, ∼0.5 μg/μL protein content) was incubated at a 1:1 mass ratio with anti-TTR antibodies 5D3 (Protego Biopharma) and PA-35315 (Invitrogen) and control anti-α-tubulin antibody DM1A (Cell Signaling Technology) for 1 h. Before TEM imaging, free antibody was removed by pelleting the amyloids and resuspension in antibody-free buffer.

Techniques: Control, Staining, Sequencing, Binding Assay